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Strategies for optimising serum-free media.

作者信息

Hewlett G

机构信息

Institute for Virology, Bayer AG, Wuppertal, Germany.

出版信息

Cytotechnology. 1991 Jan;5(1):3-14. doi: 10.1007/BF00365530.

DOI:10.1007/BF00365530
PMID:1367049
Abstract
摘要

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1
Strategies for optimising serum-free media.优化无血清培养基的策略。
Cytotechnology. 1991 Jan;5(1):3-14. doi: 10.1007/BF00365530.
2
Media for culture of mammalian cells.用于哺乳动物细胞培养的培养基。
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Carbon stripping extracts serum free fatty acids: implications for media supplementation of cultured type II pneumocytes.碳吸附法提取血清游离脂肪酸：对培养的II型肺细胞培养基补充的影响
Lab Invest. 1991 Aug;65(2):250-7.
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Human AB serum as an alternative to fetal bovine serum for endothelial and cancer cell culture.人AB血清作为内皮细胞和癌细胞培养中胎牛血清的替代品。
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Rev Neurol. 2005;40(2):69-73.
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[Defined media for hybridoma culture: do they really replace serum and why are they attractive?].[用于杂交瘤培养的限定培养基：它们真的能替代血清吗？为何具有吸引力？]
Ann Pharm Fr. 1991;49(4):198-208.
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In vitro culture of human osteosarcoma cell lines: a comparison of functional characteristics for cell lines cultured in medium without and with fetal calf serum.人骨肉瘤细胞系的体外培养：无胎牛血清培养基和含胎牛血清培养基中培养的细胞系功能特性比较
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Major cell biological parameters of keratinocytes are predetermined by culture medium and donor source.角质形成细胞的主要细胞生物学参数由培养基和供体来源预先决定。
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本文引用的文献

1
Serum-free growth medium for the cultivation of a wide spectrum of mammalian cells in stirred bioreactors.无血清生长培养基，用于搅拌式生物反应器中培养多种哺乳动物细胞。
Cytotechnology. 1988 Nov;1(4):319-29. doi: 10.1007/BF00365077.
2
Egg yolk lipoprotein, a new supplement for the growth of mammalian cells in serum-free medium : Egg yolk lipoprotein for serum-free growth of cells.蛋黄脂蛋白，一种无血清培养基中哺乳动物细胞生长的新型补充剂：无血清细胞生长用蛋黄脂蛋白。
Cytotechnology. 1988 Feb;1(2):159-69. doi: 10.1007/BF00146817.
3
Serum-free media in hybridoma culture and monoclonal antibody production.
用于Vero细胞培养的改良无血清培养基的开发：蛋白水解物、L-谷氨酰胺和SITE液体培养基补充剂对细胞生长的影响。
Cytotechnology. 2021 Oct;73(5):683-695. doi: 10.1007/s10616-020-00450-3. Epub 2021 Aug 11.
4
Selenium, Selenoproteins, and Female Reproduction: A Review.硒、硒蛋白与女性生殖：综述
Molecules. 2018 Nov 22;23(12):3053. doi: 10.3390/molecules23123053.
5
Observations on the influence of glutamine, asparagine and peptone on growth and t-PA production of Chinese hamster ovary (CHO) cells.观察谷氨酰胺、天冬酰胺和蛋白胨对中国仓鼠卵巢（CHO）细胞生长和 t-PA 产量的影响。
Cytotechnology. 1994 Jan;16(1):37-42. doi: 10.1007/BF00761777.
6
Evaluation of a simple protein free medium that supports high levels of monoclonal antibody production.评估一种简单的无蛋白培养基，该培养基能够支持高水平的单克隆抗体生产。
Cytotechnology. 1996 Jun;21(2):95-109. doi: 10.1007/BF02215660.
7
Effects of dissolved oxygen levels and the role of extra- and intracellular amino acid concentrations upon the metabolism of mammalian cell lines during batch and continuous cultures.溶解氧水平的影响以及细胞内外氨基酸浓度在分批和连续培养过程中对哺乳动物细胞系代谢的作用。
Cytotechnology. 1998 May;26(3):185-97. doi: 10.1023/A:1007917409455.
8
Short-term serum-free culture reveals that inhibition of Gsk3β induces the tumor-like growth of mouse embryonic stem cells.短期无血清培养表明，抑制 Gsk3β 会诱导小鼠胚胎干细胞的肿瘤样生长。
PLoS One. 2011;6(6):e21355. doi: 10.1371/journal.pone.0021355. Epub 2011 Jun 23.
9
Formulation of a protein-free medium based on IPL-41 for the sustained growth of Drosophila melanogaster S2 cells.基于 IPL-41 为黑腹果蝇 S2 细胞的持续生长制定无蛋白培养基。
Cytotechnology. 2008 May;57(1):11-22. doi: 10.1007/s10616-008-9153-0. Epub 2008 Jun 17.
10
The role of vitamins and amino acids on hybridoma growth and monoclonal antibody production.维生素和氨基酸对杂交瘤生长和单克隆抗体生产的作用。
Cytotechnology. 2001 Oct;37(2):65-73. doi: 10.1023/A:1019956013627.
杂交瘤培养及单克隆抗体制备中的无血清培养基。
Biotechnol Bioeng. 1988 Oct 5;32(8):1015-28. doi: 10.1002/bit.260320809.
4
Effect of pluronic F68 on growth fibroblasts in suspension on rotary shaker.
Proc Soc Exp Biol Med. 1960 Jan;103:252-4. doi: 10.3181/00379727-103-25477.
5
Protein requirements of rat ascites hepatoma cells in tissue culture.
Jpn J Exp Med. 1959 Feb;29(1):45-70.
6
Antibiotics in tissue culture.组织培养中的抗生素
Exp Cell Res. 1954 May;6(2):337-44. doi: 10.1016/0014-4827(54)90181-0.
7
Serum supplements and serum-free media: applicability for microcarrier culture of animal cells.血清补充剂和无血清培养基：在动物细胞微载体培养中的适用性。
Dev Biol Stand. 1981;50:81-91.
8
In vitro proliferation and lifespan of human diploid fibroblasts in serum-free BSA-containing medium.人二倍体成纤维细胞在含牛血清白蛋白的无血清培养基中的体外增殖及寿命
J Cell Physiol. 1982 May;111(2):155-62. doi: 10.1002/jcp.1041110206.
9
Multiplication of human diploid fibroblasts in a synthetic medium supplemented with EGF, insulin, and dexamethasone.人二倍体成纤维细胞在补充有表皮生长因子、胰岛素和地塞米松的合成培养基中的增殖。
Exp Cell Res. 1981 Aug;134(2):303-11. doi: 10.1016/0014-4827(81)90430-4.
10
Species specificity of iron delivery in hybridomas.杂交瘤中铁传递的物种特异性。
In Vitro Cell Dev Biol. 1988 May;24(5):413-9. doi: 10.1007/BF02628492.