Russell C, Mawson J, Yu P L
Department of Biotechnology, Massey University, Palmerston North, New Zealand.
Aust J Biotechnol. 1991 Jan;5(1):48-55.
This paper reviews the use of yeasts to produce heterologous proteins via the development of transformation systems. The ability to genetically engineer yeast cells has many advantages over prokaryotic systems. Yeasts are already well established in fermentation procedures, are able to secrete glycosylated and modified proteins to render the proteins biologically active, and yeasts do not secrete toxic chemicals. With the techniques available it is possible to explore yeasts as hosts for the expression and secretion of commercially-useful proteins.
本文综述了通过开发转化系统利用酵母生产异源蛋白的情况。与原核系统相比，对酵母细胞进行基因工程改造具有许多优势。酵母在发酵过程中已得到广泛应用，能够分泌糖基化和修饰后的蛋白质以使蛋白质具有生物活性，并且酵母不会分泌有毒化学物质。利用现有技术，有可能探索将酵母作为表达和分泌商业有用蛋白质的宿主。
