Park T H, Hong J, Lim H C
Biochemical Engineering Program, University of California, Irvine 92717.
Biotechnol Prog. 1991 Mar-Apr;7(2):77-84. doi: 10.1021/bp00008a001.
A cell recycle system is studied for two-stage continuous fermentation. Cell recycle around the second stage provides higher cell concentrations than processes without recycle and a longer residence time of the cell, which is necessary for inducible products, especially in recombinant cell fermentation. Residence time distribution of the cell in the fermentor is important for the optimization of inducible products. The residence time distributions are studied for the cases with and without significant cell growth in the second stage. With cell growth in the second stage, three cases are considered. These are the cases of (1) zero residence time for two daughter cells after the cell division, (2) zero residence time of one daughter cell after the cell division and inherited residence time for the other daughter cell from the mother cell after the cell division, and (3) two daughter cells having the residence time of the mother cell after the cell division.
研究了一种用于两级连续发酵的细胞循环系统。第二阶段周围的细胞循环提供了比无循环过程更高的细胞浓度和更长的细胞停留时间，这对于诱导型产物是必要的，特别是在重组细胞发酵中。细胞在发酵罐中的停留时间分布对于诱导型产物的优化很重要。研究了第二阶段有无显著细胞生长情况下的停留时间分布。对于第二阶段有细胞生长的情况，考虑了三种情形。分别是：(1) 细胞分裂后两个子细胞的停留时间为零；(2) 细胞分裂后一个子细胞的停留时间为零，另一个子细胞从母细胞继承细胞分裂后的停留时间；(3) 两个子细胞在细胞分裂后具有母细胞的停留时间。
