Kim D I, Cho G H, Pedersen H, Chin C K
Department of Chemical and Biochemical Engineering, Rutgers, State University of New Jersey, Piscataway 08855-0909.
Appl Microbiol Biotechnol. 1991 Mar;34(6):726-9. doi: 10.1007/BF00169341.
A hybrid bioreactor was developed for the production of secondary metabolites from high density cultivation of plant cell suspensions. Some of the advantages of both air-lift and cell-lift by agitation were combined. The addition of a decanting column also made it possible to run a perfusion system for high density culture or to run a two-stage culture efficiently. Cell growth and the production of berberine from Thalictrum rugosum in the hybrid bioreactor are reported in this paper. A cell density up to 31 g/l was obtained by perfusion without any problems in mixing or loss of cell viability and the specific berberine productivity was comparable to that in shake flasks. The maximum berberine concentration was 88 mg/l at 3 weeks of operation and declined thereafter.
开发了一种用于从植物细胞悬浮液的高密度培养中生产次生代谢产物的混合生物反应器。气升式和搅拌式细胞提升的一些优点被结合在一起。倾析柱的添加还使得运行用于高密度培养的灌注系统或高效运行两阶段培养成为可能。本文报道了在混合生物反应器中毛茛细胞的生长和小檗碱的生产情况。通过灌注获得了高达31 g/l的细胞密度，混合或细胞活力丧失方面没有任何问题，并且小檗碱的比生产率与摇瓶中的相当。在运行3周时，小檗碱的最大浓度为88 mg/l，此后下降。
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