Kuo Yu-Chieh, Tan Chia-Chun, Ku Jung-Ting, Hsu Wei-Cho, Su Sung-Chieh, Lu Chung-An, Huang Li-Fen
Graduate School of Biotechnology and Bioengineering, Yuan Ze University, 135 Yuan-Tung Road, Taoyuan 32003, Taiwan.
Int J Mol Sci. 2013 Apr 24;14(5):8719-39. doi: 10.3390/ijms14058719.
Application of plant expression systems in the production of recombinant proteins has several advantages, such as low maintenance cost, absence of human pathogens, and possession of complex post-translational glycosylation capabilities. Plants have been successfully used to produce recombinant cytokines, vaccines, antibodies, and other proteins, and rice (Oryza sativa) is a potential plant used as recombinant protein expression system. After successful transformation, transgenic rice cells can be either regenerated into whole plants or grown as cell cultures that can be upscaled into bioreactors. This review summarizes recent advances in the production of different recombinant protein produced in rice and describes their production methods as well as methods to improve protein yield and quality. Glycosylation and its impact in plant development and protein production are discussed, and several methods of improving yield and quality that have not been incorporated in rice expression systems are also proposed. Finally, different bioreactor options are explored and their advantages are analyzed.
植物表达系统在重组蛋白生产中的应用具有若干优势，如维护成本低、不存在人类病原体以及具备复杂的翻译后糖基化能力。植物已成功用于生产重组细胞因子、疫苗、抗体及其他蛋白质，而水稻（Oryza sativa）是用作重组蛋白表达系统的一种潜在植物。成功转化后，转基因水稻细胞既可以再生为完整植株，也可以作为细胞培养物生长，进而扩大规模用于生物反应器。本综述总结了水稻中生产不同重组蛋白的最新进展，描述了它们的生产方法以及提高蛋白产量和质量的方法。讨论了糖基化及其对植物发育和蛋白生产的影响，还提出了几种尚未纳入水稻表达系统的提高产量和质量的方法。最后，探讨了不同的生物反应器选择并分析了它们的优势。
