Lee J, Agathos S N
Department of Chemical and Biochemical Engineering, State University of New Jersey, Piscataway 08855-0909.
Appl Microbiol Biotechnol. 1991 Jan;34(4):513-7. doi: 10.1007/BF00180580.
We report the kinetics of endogenous L-valine in the fungus Tolypocladium inflatum, in an effort to understand the enhancing effect of externally supplemented L-valine on the production of the immunosuppressant cyclosporin A (CyA) in chemically defined medium. In a batch laboratory stirred reactor cultivation, the concentration of intracellular L-valine increased by up to four times between the end of the exponential phase and the beginning of the stationary phase when the medium was supplemented externally with 4 g/l L-valine. The final CyA titre under these conditions was 710 mg/l compared to only 130 mg/l attained without L-valine supplementation. In contrast to substantial growth-associated production of CyA in unsupplemented culture, the formation of the immunosuppressant was prolonged during the stationary phase in L-valine-supplemented medium. As a result, the conversion yield of CyA on L-valine remained constant during the stationary phase at 0.27 g CyA/g L-valine.
我们报告了真菌顶头孢霉中内源性L-缬氨酸的动力学，旨在了解在化学成分确定的培养基中，外源添加L-缬氨酸对免疫抑制剂环孢菌素A（CyA）产量的增强作用。在实验室分批搅拌反应器培养中，当培养基中外源添加4 g/L L-缬氨酸时，指数期末期至稳定期初期，细胞内L-缬氨酸浓度增加了四倍。在这些条件下，最终的CyA产量为710 mg/L，而未添加L-缬氨酸时仅为130 mg/L。与未添加培养基中与大量生长相关的CyA产生不同，在添加L-缬氨酸的培养基中，免疫抑制剂的形成在稳定期延长。因此，在稳定期，CyA对L-缬氨酸的转化率保持恒定，为0.27 g CyA/g L-缬氨酸。
