Institut für Biochemie der Technischen Universität Berlin, Franklinstrasse 29, D-1000 Berlin 10 (West), Federal Republic of Germany.
Appl Environ Microbiol. 1988 Oct;54(10):2504-9. doi: 10.1128/aem.54.10.2504-2509.1988.
The production of enniatins by Fusarium scirpi during fermentative growth in submerged cultures was measured. The fungus produced the antibiotic during mycelial growth, but not during the stationary phase of cultivation. By contrast, enniatin synthetase, the enzyme responsible for enniatin synthesis, was present during growth, during the stationary phase, and even in spores. Similarly, the enniatin synthetase mRNA was present at every stage of the cultivation of the fungus. Therefore, this multifunctional peptide synthetase is a constitutive enzyme, the expression of which is not regulated by any specific mechanism. The findings stand in contrast to the common assumption that production of secondary metabolites underlies regulatory control, leading to separation of the trophophase and the idiophase.
研究了尖孢镰刀菌在液体发酵培养过程中的产烯菌胺情况。该真菌在菌丝生长过程中产生抗生素，但在培养的静止期不产生。相比之下，烯菌胺合成酶，即负责烯菌胺合成的酶，在生长过程中、静止期甚至在孢子中都存在。同样，烯菌胺合成酶 mRNA 在真菌培养的各个阶段都存在。因此，这种多功能肽合成酶是一种组成型酶，其表达不受任何特定机制的调节。这一发现与普遍假设的次生代谢产物的产生受调控控制的观点相反，该观点导致了营养期和静止期的分离。
