利用“体外”胚胎生产技术培育转基因奶牛。

Generation of transgenic dairy cattle using 'in vitro' embryo production.

作者信息

Krimpenfort P, Rademakers A, Eyestone W, van der Schans A, van den Broek S, Kooiman P, Kootwijk E, Platenburg G, Pieper F, Strijker R

机构信息

Department of Embryology, Gene Pharming Europe B.V., Leiden, The Netherlands.

出版信息

Biotechnology (N Y). 1991 Sep;9(9):844-7. doi: 10.1038/nbt0991-844.

DOI:10.1038/nbt0991-844

PMID:1367358

Abstract

We have combined gene transfer, by microinjection, with 'in vitro' embryo production technology, enabling us to carry out non-surgical transfer, to recipient cows, of microinjected embryos that have been cultured from immature oocytes. Using this approach, we have established 21 pregnancies from which 19 calves were born. Southern blot analysis proved that in two cases the microinjected DNA had been integrated in the host genome.

摘要

我们将通过显微注射进行的基因转移与“体外”胚胎生产技术相结合，从而能够将从未成熟卵母细胞培养而来的显微注射胚胎非手术移植到受体母牛体内。采用这种方法，我们已成功建立了21次妊娠，产下了19头小牛。Southern印迹分析证明，在两例中，显微注射的DNA已整合到宿主基因组中。