Mohan S B, Lyddiatt A
School of Chemical Engineering, University of Birmingham, UK.
Cytotechnology. 1991 Mar;5(3):201-9. doi: 10.1007/BF00556290.
Evidence is presented for the passive release of monoclonal antibodies (MCAB) from hybridoma cells grown in either batch or continuous-flow culture. This release is promoted at room temperature. Passively released MCAB is indistinguishable from that released by actively growing cells, as judged by SDS-polyacrylamide gel electrophoresis. The significance of these observations in relation to the continuous culture of hybridoma cells is discussed. Maximum MCAB content of TB/C3 hybridoma cells is about 55pg per cell, any additional MCAB produced is secreted.
有证据表明，在分批培养或连续流培养中生长的杂交瘤细胞会被动释放单克隆抗体（MCAB）。这种释放在室温下会加速。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳判断，被动释放的MCAB与活跃生长细胞释放的MCAB没有区别。讨论了这些观察结果与杂交瘤细胞连续培养的相关性。TB/C3杂交瘤细胞的最大MCAB含量约为每个细胞55皮克，产生的任何额外MCAB都会分泌出来。
