Lawton L A, Campbell D L, Beattie K A, Codd G A
Department of Biological Sciences, University of Dundee, UK.
Lett Appl Microbiol. 1990 Oct;11(4):205-7. doi: 10.1111/j.1472-765x.1990.tb00161.x.
The recent rise in the awareness of the occurrence of toxic cyanobacterial blooms in aquatic environments, with associated human health problems and animal deaths, has increased the need for rapid, reliable and sensitive methods of determining cyanobacterial toxicity. A luminescent bacterial toxicity test was assessed as a complement to the established mouse bioassay. Seventeen samples including pure cyanobacterial microcystin-LR hepatotoxin, laboratory isolates and natural blooms of cyanobacteria were tested and toxicity data compared with mouse LD50 values. Microcystin-LR and all five microcystin-containing cyanobacterial samples, hepatotoxic by mouse test gave EC50 values of less than 0.46 mg/ml in bioluminescence-based Microtox assays. Of 11 samples non-toxic by mouse bioassay, only two gave an EC50 of less than 0.98 mg/ml by bioluminescence assay. It is suggested that the Microtox bioluminescence assay may prove useful in the preliminary screening of cyanobacterial blooms for microcystin-based toxicity.
近期，人们对水生环境中有毒蓝藻水华的发生及其引发的人类健康问题和动物死亡的认识有所提高，这使得对快速、可靠且灵敏的蓝藻毒性测定方法的需求增加。一种发光细菌毒性测试被评估为对既定小鼠生物测定法的补充。对17个样本进行了测试，包括纯蓝藻微囊藻毒素-LR肝毒素、实验室分离株和天然蓝藻水华，并将毒性数据与小鼠半数致死量（LD50）值进行了比较。微囊藻毒素-LR以及所有五个含微囊藻毒素的蓝藻样本，经小鼠试验具有肝毒性，在基于生物发光的Microtox测定中，其半数有效浓度（EC50）值小于0.46毫克/毫升。在11个经小鼠生物测定无毒的样本中，只有两个通过生物发光测定的EC50小于0.98毫克/毫升。有人认为，Microtox生物发光测定法可能在基于微囊藻毒素的毒性对蓝藻水华进行初步筛选中有用。
