葡萄球菌中毒性休克综合征毒素-1（TSST-1）基因的筛查。
Screening of staphylococci for the toxic shock syndrome toxin-1 (TSST-1) gene.
作者信息
Jaulhac B, De Buyser M L, Dilasser F, Prevost G, Piedmont Y
机构信息
Institut de Bactériologie, Université Louis Pasteur, Strasbourg, France.
出版信息
Lett Appl Microbiol. 1991 Aug;13(2):90-2. doi: 10.1111/j.1472-765x.1991.tb00578.x.
Dot blot hybridization was used to screen 820 staphylococci for the presence of the gene coding for TSST-1. The DNA of 33 strains among 70 Staph. aureus strains isolated from suspected toxic shock syndrome (TSS) cases hybridized with the probe. These results agreed perfectly with those obtained with a phenotypic method (immunodiffusion). Among 608 Staph. aureus strains isolated over a period of one month from hospitalized patients, 66 (11%) hybridized with the probe; of these strains, 64 (97%) were found to produce TSST-1 in vitro. None of 145 coagulase-negative staphylococcal strains harboured DNA hybridizing with the probe. The data indicate that this genotypic assay is suitable for epidemiological studies.
采用斑点印迹杂交法对820株葡萄球菌进行检测，以筛查编码中毒性休克综合征毒素-1（TSST-1）的基因。从疑似中毒性休克综合征（TSS）病例中分离出的70株金黄色葡萄球菌中，有33株的DNA与探针杂交。这些结果与用表型方法（免疫扩散法）获得的结果完全一致。在从住院患者中历时1个月分离出的608株金黄色葡萄球菌中，有66株（11%）与探针杂交；在这些菌株中，有64株（97%）在体外被发现可产生TSST-1。145株凝固酶阴性葡萄球菌菌株中无一株含有与探针杂交的DNA。数据表明，这种基因分型检测方法适用于流行病学研究。
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