Parsonnet J, Harrison A E, Spencer S E, Reading A, Parsonnet K C, Kass E H
J Clin Microbiol. 1987 Aug;25(8):1370-2. doi: 10.1128/jcm.25.8.1370-1372.1987.
We tested 187 strains of coagulase-negative staphylococci (CNS) for the production of toxic shock syndrome toxin 1 (TSST-1). A total of 111 CNS strains were isolated from the tampons of menstruating women and 74 were isolated from unused tampons. Two strains were isolated from the genital tract of a patient with toxic shock syndrome. Strains were cultivated by the membrane-over-agar method to enhance production of TSST-1, and culture supernatants were tested by two exquisitely sensitive enzyme-linked immunosorbent assays. None of the 187 CNS strains produced TSST-1. We conclude that CNS colonizing the genital tracts of menstruating women and unused tampons produce TSST-1 infrequently, if ever, and are unlikely to play a role in toxic shock syndrome.
我们检测了187株凝固酶阴性葡萄球菌(CNS)是否产生中毒性休克综合征毒素1(TSST-1)。总共从月经女性的卫生棉条中分离出111株CNS菌株,从未使用过的卫生棉条中分离出74株。从一名中毒性休克综合征患者的生殖道中分离出两株。采用琼脂覆盖膜法培养菌株以增强TSST-1的产生,并通过两种极其灵敏的酶联免疫吸附试验检测培养上清液。187株CNS菌株均未产生TSST-1。我们得出结论,定殖于月经女性生殖道和未使用过的卫生棉条上的CNS极少产生TSST-1,即便产生也不太可能在中毒性休克综合征中起作用。