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荧光原位杂交后染色体显带的一种改进方法：使用四甲基罗丹明偶联的BrdU抗体。

An improved method for chromosome banding after fluorescence in situ hybridization: use of a tetramethylrhodhamine-conjugated BrdU antibody.

作者信息

De Sario A, De Carli L, Raimondi E

机构信息

Dipartimento di Genetica e Microbiologia, A. Buzzati-Traverso, Pavia, Italy.

出版信息

Cytotechnology. 1991 Sep;7(1):55-8. doi: 10.1007/BF00135639.

DOI:10.1007/BF00135639
PMID:1367701
Abstract

A method for high quality chromosome banding after in situ hybridization with biotinylated probes has been developed. Fluoresceine-conjugated avidin is used for probe detection, while chromosome banding is performed with a tetramethylrodhamine-conjugated anti-BrdU antibody. In this way probe localization and chromosome identification can be performed simultaneously simply by changing the incidental light wavelength.

摘要

已开发出一种在与生物素化探针原位杂交后进行高质量染色体显带的方法。用荧光素偶联抗生物素蛋白进行探针检测，而用四甲基罗丹明偶联抗 BrdU 抗体进行染色体显带。通过这种方式，只需改变入射光波长，即可同时进行探针定位和染色体识别。

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An improved method for chromosome banding after fluorescence in situ hybridization: use of a tetramethylrhodhamine-conjugated BrdU antibody.荧光原位杂交后染色体显带的一种改进方法：使用四甲基罗丹明偶联的BrdU抗体。
Cytotechnology. 1991 Sep;7(1):55-8. doi: 10.1007/BF00135639.
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引用本文的文献

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Simultaneous detection of FISH signals and bromo-deoxyuridine incorporation in fixed tissue cultured cells.在固定的组织培养细胞中同时检测荧光原位杂交（FISH）信号和溴脱氧尿苷掺入情况。
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本文引用的文献

1
New Giemsa method for the differential staining of sister chromatids.用于姐妹染色单体鉴别染色的新吉姆萨方法。
Nature. 1974 Sep 13;251(5471):156-8. doi: 10.1038/251156a0.
2
Isolation and characterization of an alphoid centromeric repeat family from the human Y chromosome.从人类Y染色体中分离和鉴定一个α卫星着丝粒重复序列家族。
J Mol Biol. 1985 Apr 20;182(4):477-85. doi: 10.1016/0022-2836(85)90234-7.
3
DNA probe localization at 18p113 band by in situ hybridization and identification of a small supernumerary chromosome.通过原位杂交将DNA探针定位在18p11.3带，并鉴定出一条小的额外染色体。
Hum Genet. 1985;69(3):268-71. doi: 10.1007/BF00293038.
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Translocation of c-abl to "masked" Ph in chronic myeloid leukemia.慢性粒细胞白血病中c-abl易位至“隐匿”的费城染色体。
Cancer Genet Cytogenet. 1985 Oct;18(2):95-104. doi: 10.1016/0165-4608(85)90059-7.
5
Regional mapping of the human placental alkaline phosphatase gene (ALPP) to 2q37 by in situ hybridization.通过原位杂交将人胎盘碱性磷酸酶基因（ALPP）定位到2q37区域。
Cytogenet Cell Genet. 1988;47(1-2):98-9. doi: 10.1159/000132518.
6
Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries.利用重组DNA文库通过原位抑制杂交技术描绘中期和间期细胞中的单个人类染色体。
Hum Genet. 1988 Nov;80(3):224-34. doi: 10.1007/BF01790090.
7
Sensitive, high-resolution chromatin and chromosome mapping in situ: presence and orientation of two closely integrated copies of EBV in a lymphoma line.敏感、高分辨率的染色质和染色体原位图谱：淋巴瘤细胞系中两个紧密整合的EBV拷贝的存在及方向
Cell. 1988 Jan 15;52(1):51-61. doi: 10.1016/0092-8674(88)90530-2.
8
Mapping of single-copy DNA sequences on human chromosomes by in situ hybridization with biotinylated probes: enhancement of detection sensitivity by intensified-fluorescence digital-imaging microscopy.通过与生物素化探针进行原位杂交将单拷贝DNA序列定位到人类染色体上：利用增强荧光数字成像显微镜提高检测灵敏度。
Proc Natl Acad Sci U S A. 1989 Jan;86(2):582-6. doi: 10.1073/pnas.86.2.582.
9
Detection of BrdUrd incorporation in mammalian chromosomes by a BrdUrd antibody. III. Demonstration of replication patterns in highly resolved chromosomes.利用BrdUrd抗体检测哺乳动物染色体中的BrdUrd掺入。III. 高分辨率染色体复制模式的展示。
Hum Genet. 1987 Jul;76(3):293-5. doi: 10.1007/BF00283627.
10
Detection of bromodeoxyuridine-incorporation in mammalian chromosomes by a bromodeoxyuridine-antibody. I. Demonstration of replication patterns.通过溴脱氧尿苷抗体检测哺乳动物染色体中的溴脱氧尿苷掺入。I. 复制模式的证明
Hum Genet. 1986 Feb;72(2):129-32. doi: 10.1007/BF00283930.