De Sario A, De Carli L, Raimondi E
Dipartimento di Genetica e Microbiologia, A. Buzzati-Traverso, Pavia, Italy.
Cytotechnology. 1991 Sep;7(1):55-8. doi: 10.1007/BF00135639.
A method for high quality chromosome banding after in situ hybridization with biotinylated probes has been developed. Fluoresceine-conjugated avidin is used for probe detection, while chromosome banding is performed with a tetramethylrodhamine-conjugated anti-BrdU antibody. In this way probe localization and chromosome identification can be performed simultaneously simply by changing the incidental light wavelength.
已开发出一种在与生物素化探针原位杂交后进行高质量染色体显带的方法。用荧光素偶联抗生物素蛋白进行探针检测，而用四甲基罗丹明偶联抗 BrdU 抗体进行染色体显带。通过这种方式，只需改变入射光波长，即可同时进行探针定位和染色体识别。
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