Lantsov A A, Il'inskaia E V
Vestn Otorinolaringol. 2003(4):8-11.
The experiments were made on guinea pigs injected i.m. kanamycin for 7 and 14 days in a dose 400 mg/kg. The cochleas of the guinea pigs were studied electron microscopically after 7- and 14-day courses of the antibiotic and 30 days after the end of aminoglycoside administration. To assess the vascular stripe (VS) function, the number of secretory vacuoles in apical cytoplasm of the marginal cells on the ultrathin sections was calculated. The animals which had got kanamycin for 7 days exhibited insignificant VS ultrastructural alterations, the number of vacuoles in the marginal cells (38.9 +/- 2.4) was higher than normal (33.9 +/- 1.5) but the difference was insignificant. After 14-day administration of the antibiotic VS of the animals had noticeable ultrastructural disorders of the interstitial and marginal cells and statistically significant (p < 0.01) suppression of secretory activity of the marginal cells (18.6 +/- 1.1). Natural reparative processes normalized ultrastructure of all types of VS cells 30 days after kanamycin discontinuation though secretion in the marginal cells remained significantly subnormal (the number of the vacuoles reduced to 23.4 +/- 1.7). Thus, aminoglycoside antibiotic kanamycin induces morphofunctional disorders in VS cells which do not return to normal after 30 days of follow-up.
实验选用豚鼠，肌肉注射卡那霉素，剂量为400mg/kg，持续7天和14天。在使用抗生素7天和14天疗程后以及氨基糖苷类药物给药结束后30天，对豚鼠的耳蜗进行电子显微镜研究。为评估血管纹（VS）功能，计算超薄切片上边缘细胞顶端细胞质中分泌泡的数量。接受卡那霉素治疗7天的动物，其VS超微结构改变不明显，边缘细胞中的泡数量（38.9±2.4）高于正常水平（33.9±1.5），但差异不显著。抗生素给药14天后，动物的VS出现明显的间质细胞和边缘细胞超微结构紊乱，边缘细胞分泌活性受到统计学显著抑制（p<0.01）（18.6±1.1）。停用卡那霉素30天后，自然修复过程使所有类型的VS细胞超微结构恢复正常，尽管边缘细胞的分泌仍明显低于正常水平（泡数量减少至23.4±1.7）。因此，氨基糖苷类抗生素卡那霉素可诱导VS细胞发生形态功能障碍，随访30天后仍未恢复正常。
