源自16S rRNA的属特异性和种特异性寡核苷酸探针用于阴道球菌的鉴定。
Genus- and species-specific oligonucleotide probes derived from 16S rRNA for the identification of vagococci.
作者信息
Williams A M, Collins M D
机构信息
Department of Microbiology, AFRC Institute of Food Research, Shinfield, Reading, UK.
出版信息
Lett Appl Microbiol. 1992 Feb;14(2):17-21. doi: 10.1111/j.1472-765x.1992.tb00637.x.
Abstract
Synthetic oligonucleotide probes specific for vagococci were designed from 16S rRNA sequence data. Molecular hybridizations with PCR-amplified rDNA targets provided an unequivocal means of differentiating vagococci from related lactic acid bacteria (eg. Carnobacterium, Enterococcus, Lactococcus) and identification at the generic and species levels.
摘要
根据16S rRNA序列数据设计了针对漫游球菌的合成寡核苷酸探针。与PCR扩增的rDNA靶标进行分子杂交，为区分漫游球菌与相关乳酸菌（如肉杆菌属、肠球菌属、乳球菌属）提供了明确的方法，并能在属和种水平上进行鉴定。
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