利用聚合酶链反应扩增的16S rRNA可变区和特异性DNA探针鉴定嗜温乳酸菌。
Identification of mesophilic lactic acid bacteria by using polymerase chain reaction-amplified variable regions of 16S rRNA and specific DNA probes.
作者信息
Klijn N, Weerkamp A H, de Vos W M
机构信息
Netherlands Institute for Dairy Research (NIZO), Ede.
出版信息
Appl Environ Microbiol. 1991 Nov;57(11):3390-3. doi: 10.1128/aem.57.11.3390-3393.1991.
Abstract
Specific DNA probes based on variable regions V1 and V3 of 16S rRNA of lactic acid bacteria were designed. These probes were used in hybridization experiments with variable regions amplified by using the polymerase chain reaction. In this way, a rapid and sensitive method was developed for the identification and classification of Lactococcus and Leuconostoc species.
摘要
设计了基于乳酸菌16S rRNA可变区V1和V3的特异性DNA探针。这些探针用于与通过聚合酶链反应扩增的可变区进行杂交实验。通过这种方式，开发了一种快速灵敏的方法用于乳酸球菌属和明串珠菌属菌种的鉴定和分类。
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