Chromosome microtechnology: microdissection and microcloning.

The physical microdissection of chromosomes and subsequent microcloning of dissected fragments is enabling the generation of very large numbers of cloned unique sequences from defined chromosomal regions. In addition to use in constructing region-specific libraries of the entire human genome and providing probes for mapping and sequencing purposes, such chromosome microtechnology should facilitate the search for disease-associated genes in defined chromosome regions.