用于细胞内蛋白质检测的单金属纳米颗粒成像
Single metallic nanoparticle imaging for protein detection in cells.
作者信息
Cognet L, Tardin C, Boyer D, Choquet D, Tamarat P, Lounis B
机构信息
Centre de Physique Moléculaire Optique et Hertzienne, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5798 et Université Bordeaux 1, 351 Cours de la Libération, 33405 Talence, France.
出版信息
Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11350-5. doi: 10.1073/pnas.1534635100. Epub 2003 Sep 17.
Abstract
We performed a visualization of membrane proteins labeled with 10-nm gold nanoparticles in cells, using an all-optical method based on photothermal interference contrast. The high sensitivity of the method and the stability of the signals allows 3D imaging of individual nanoparticles without the drawbacks of photobleaching and blinking inherent to fluorescent markers. A simple analytical model is derived to account for the measurements of the signal amplitude and the spatial resolution. The photothermal interference contrast method provides an efficient, reproducible, and promising way to visualize low amounts of proteins in cells by optical means.
摘要
我们使用基于光热干涉对比度的全光学方法，对细胞中用10纳米金纳米颗粒标记的膜蛋白进行了可视化。该方法的高灵敏度和信号稳定性使得能够对单个纳米颗粒进行三维成像，而没有荧光标记固有的光漂白和闪烁缺点。推导了一个简单的分析模型来解释信号幅度和空间分辨率的测量结果。光热干涉对比度方法为通过光学手段可视化细胞中少量蛋白质提供了一种高效、可重复且有前景的方法。
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