Choi Seong-Ho, Hwang Young-Mi, Ryoo Jae Jeong, Lee Kwang-Pill, Ohta Kazutoku, Takeuchi Toyohide, Jin Ji-Ye, Fujimoto Chuzo
Department of Chemistry Graduate School, Kyungpook National University, Daegu, South Korea.
Electrophoresis. 2003 Sep;24(18):3181-6. doi: 10.1002/elps.200305581.
Surface grafting of glycidyl methacrylate (GMA) on silica gel and a polyethylene bead was performed by radical polymerization and radiation-induced polymerization, respectively, in order to improve softness. Subsequently, diethylene triamine (DETA), triethylene tetraamine (TETA), and iminodiacetic acid (IDA) were introduced to the grafted GMA for use as affinity columns. The efficiency of the affinity column was investigated by use of bovine serum albumin (BSA) and hemoglobin (Hb) as model proteins. The affinity degree of BSA was higher than Hb for the DETA and TETA column, whereas the affinity degree of Hb was higher than BSA for the IDA column supported by silica gel. The affinity degree of BSA was higher than Hb for the DETA and TTA column supported by polyethylene (PE) beads.
为了提高柔软度，分别通过自由基聚合和辐射诱导聚合将甲基丙烯酸缩水甘油酯（GMA）接枝到硅胶和聚乙烯珠粒表面。随后，将二亚乙基三胺（DETA）、三亚乙基四胺（TETA）和亚氨基二乙酸（IDA）引入到接枝的GMA上，用作亲和柱。以牛血清白蛋白（BSA）和血红蛋白（Hb）作为模型蛋白，研究了亲和柱的效率。对于DETA和TETA柱，BSA的亲和度高于Hb，而对于硅胶负载的IDA柱，Hb的亲和度高于BSA。对于聚乙烯（PE）珠粒负载的DETA和TTA柱，BSA的亲和度高于Hb。
