Huaracha E, Xu M, Korban S S
Department of Natural Resources and Environmental Sciences, University of Illinois, 310 Madigan Building, 1201 West Gregory Drive, Urbana, IL 97331, USA.
Theor Appl Genet. 2004 Jan;108(2):274-9. doi: 10.1007/s00122-003-1435-5. Epub 2003 Sep 6.
A narrow-down strategy to restrict the Vf region, which controls resistance to the fungal disease apple scab in apple, to a genetic distance of 0.4 cM is presented. Using 11 AFLP-derived SCARs and three RAPD-derived SCARs, all linked to the Vf gene, we subjected 1,412 scab-resistant individuals from 16 mapping populations to genotype analysis. Eleven recombinant individuals were identified within a genetic distance of 0.9 cM around the Vf gene. Using these 11 recombinants, we achieved fine-resolution of several AFLP-derived SCAR markers surrounding the Vf gene, resulting in the following genetic linkage map: ACS-6 and ACS are located left of the Vf gene at genetic distances of 0.2 cM and 0.1 cM, respectively; ACS-7 and ACS-9 are inseparable from the Vf gene; ACS-8, ACS-10, and ACS-4 are located to the right of the Vf gene at genetic distances of 0.1 cM, 0.4 cM, and 0.5 cM, respectively; the remaining five SCARs-ACS-11, ACS-5, ACS-2, ACS-1, and AL07-are inseparable and are located right of the Vf gene at a genetic distance of 0.7 cM. By integrating this linkage data with our previous physical map, we generated a revised map of the narrowed-down region of Vf.
本文提出了一种将控制苹果对真菌病害苹果黑星病抗性的Vf区域限制在0.4厘摩遗传距离内的缩小策略。利用11个与Vf基因连锁的AFLP衍生SCAR标记和3个RAPD衍生SCAR标记，我们对来自16个作图群体的1412个抗黑星病个体进行了基因型分析。在Vf基因周围0.9厘摩的遗传距离内鉴定出11个重组个体。利用这11个重组体，我们对Vf基因周围的几个AFLP衍生SCAR标记进行了精细定位，得到了如下遗传连锁图谱：ACS-6和ACS分别位于Vf基因左侧，遗传距离为0.2厘摩和0.1厘摩；ACS-7和ACS-9与Vf基因不可分离；ACS-8、ACS-10和ACS-4分别位于Vf基因右侧，遗传距离为0.1厘摩、0.4厘摩和0.5厘摩；其余5个SCAR标记——ACS-11、ACS-5、ACS-2、ACS-1和AL07——不可分离，位于Vf基因右侧，遗传距离为0.7厘摩。通过将这些连锁数据与我们之前的物理图谱整合，我们生成了Vf缩小区域的修订图谱。
