Department of Horticulture, University of Illinois, 310 Madigan Bldg., 1201 W. Gregory Drive, 61801, Urbana, IL, USA.
Theor Appl Genet. 1996 Feb;92(2):263-6. doi: 10.1007/BF00223383.
Apple scab, caused by Venturia inaequalis (Cke.) Wint., is the most serious disease of apple trees in many areas of the world. Resistance to V. inaequalis, derived from the small-fruited species Malus floribunda 821, is determined by a major dominant gene, Vf. Using random decamer primers, we identified a RAPD marker, OPD20/600, which is linked to the Vf gene. OPD20/600 was then cloned and sequenced. Sequence-specific primers based on the marker were used to further screen M. floribunda 821, 7 scab-susceptible apple cultivars, 10 scab-resistant apple cultivars, and 28 scab-resistant Coop selections. The sequence-specific primers allowed identification of polymorphisms of OPD20/600 based on the presence or absence of a single band. The advantages of sequence-specific primers over decamer primers for developing genetic markers are discussed.
苹果黑星病，由梨黑星病菌（Venturia inaequalis）引起，是世界上许多地区苹果树面临的最严重病害。从小果型种苹果绵蚜（Malus floribunda）821 中获得的对梨黑星病菌的抗性，由一个主效显性基因 Vf 决定。我们使用随机十聚体引物，鉴定出与 Vf 基因连锁的 RAPD 标记 OPD20/600。随后对 OPD20/600 进行了克隆和测序。基于该标记的序列特异性引物被进一步用于筛选小果型种苹果绵蚜 821、7 个苹果品种易感黑星病、10 个苹果品种抗黑星病以及 28 个苹果抗性 Coop 系。序列特异性引物能够根据单条带的有无鉴定 OPD20/600 的多态性。讨论了与十聚体引物相比，序列特异性引物在开发遗传标记方面的优势。
