Isobe S, Klimenko I, Ivashuta S, Gau M, Kozlov N N
National Agricultural Research Center for Hokkaido Region, Hitsujigaoka 1, Toyohira, Sapporo, 062-8555, Hokkaido, Japan.
Theor Appl Genet. 2003 Dec;108(1):105-12. doi: 10.1007/s00122-003-1412-z. Epub 2003 Sep 16.
We constructed a genetic linkage map of red clover ( Trifolium pratense L., 2n=2 x=14) using RFLP markers from cDNA probes of a backcrossed mapping population, and investigated the transferability of the markers to other red clover germplasm. The map contains 157 RFLP markers and one morphological marker on seven linkage groups. The total map distance was 535.7 cM and the average distance between two markers was 3.4 cM. All of the cDNA probes of the map were hybridized to the fragments of genomic DNA from 12 plants derived from three varieties, and 87% of the cDNA probes detected polymorphic bands that corresponded to those of mapping parents. This result indicated that RFLP markers on the present map were transferable to the genome analysis of other red clover germplasm. This is the first report to construct a linkage map of Trifolium species; it should provide fundamental and useful genetic information relevant to the breeding of red clover and genus Trifolium.
我们利用回交作图群体的cDNA探针的RFLP标记构建了红三叶草（Trifolium pratense L.，2n = 2x = 14）的遗传连锁图谱，并研究了这些标记向其他红三叶草种质的可转移性。该图谱在7个连锁群上包含157个RFLP标记和1个形态标记。图谱总长度为535.7 cM，两个标记之间的平均距离为3.4 cM。图谱上所有的cDNA探针都与来自三个品种的12株植物的基因组DNA片段杂交，87%的cDNA探针检测到与作图亲本相对应的多态性条带。这一结果表明，本图谱上的RFLP标记可转移用于其他红三叶草种质的基因组分析。这是构建三叶草属物种连锁图谱的首次报道；它应为红三叶草和三叶草属的育种提供基础且有用的遗传信息。
