利用 bulked 基因组 DNA 样品随机扩增多态性技术快速估计不同苜蓿群体间的遗传关系。
Rapid estimation of genetic relatedness among heterogeneous populations of alfalfa by random amplification of bulked genomic DNA samples.
机构信息
Crop Science Department, University of Guelph, NIG 2W1, Guelph, Ontario, Canada.
出版信息
Theor Appl Genet. 1993 Jul;86(6):788-94. doi: 10.1007/BF00222671.
A procedure which involves the use of RAPD markers, obtained from bulked genomic DNA samples, to estimate genetic relatedness among heterogeneous populations is demonstrated in this study. Bulked samples of genomic DNA from several alfalfa plants per population were used as templates in polymerase chain reactions with different random primers to produce RAPD patterns. The results show that the RAPD patterns can be used to determine genetic distances among heterogeneous populations and cultivars which correspond to their known relatedness. The results also indicate that, by using ten primers with bulked DNA samples from ten individuals, 18-72 populations or cultivars can be distinguished from each other on the basis of at least one unique RAPD marker. We anticipate that DNA bulking and methods for comparing RAPD patterns will be very useful for identifying cultivars, for studying phylogenetic relationships among heterogeneous populations and for selecting parents to maximize heterosis in crosses.
本研究展示了一种使用 RAPD 标记（从混合基因组 DNA 样本中获得）来估计异质群体间遗传关系的方法。每个群体的多个紫花苜蓿植物的混合基因组 DNA 样本被用作聚合酶链反应中的模板，用不同的随机引物产生 RAPD 图谱。结果表明，RAPD 图谱可用于确定异质群体和品种之间的遗传距离，这与其已知的亲缘关系相对应。结果还表明，使用十个随机引物对来自十个个体的混合 DNA 样本进行分析，至少可以根据一个独特的 RAPD 标记将 18-72 个群体或品种区分开来。我们预计，DNA 混合和比较 RAPD 图谱的方法将非常有助于鉴定品种，研究异质群体间的系统发育关系，以及选择亲本以最大限度地提高杂交中的杂种优势。
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