R Tormo José, García Juan B, DeAntonio Mar, Feliz Julia, Mira Aurora, Díez M Teresa, Hernández Pilar, Peláez Fernando
Centro de Investigación Básica, Merck Research Laboratories, Merck, Sharp & Dohme de España S.A., C/Josefa Valcárcel 38, 28027, Madrid, Spain.
J Ind Microbiol Biotechnol. 2003 Oct;30(10):582-8. doi: 10.1007/s10295-003-0084-7. Epub 2003 Sep 9.
The manipulation of growth conditions of microorganisms is a common strategy used by pharmaceutical companies to improve the quantities and spectra of secondary metabolites with potential therapeutic interest. In this work, the effects of fermentation media on secondary metabolite production from a set of Actinomycetes was statistically compared. For this purpose, we created an automated method for comparing the ability of microorganisms to produce different secondary metabolites. HPLC analyses guided the selection of those media in which a wider chemical diversity was obtained from microorganisms inoculated in a wide spectrum of production media. Fermented media yielding a better secondary metabolite profile were included in subsequent drug discovery screening.
操纵微生物的生长条件是制药公司常用的一种策略，用于提高具有潜在治疗价值的次级代谢产物的产量和种类。在这项工作中，我们对发酵培养基对一组放线菌次级代谢产物生产的影响进行了统计学比较。为此，我们创建了一种自动化方法，用于比较微生物产生不同次级代谢产物的能力。高效液相色谱分析指导了对那些能从接种于多种生产培养基中的微生物获得更广泛化学多样性的培养基的选择。产生更好次级代谢产物谱的发酵培养基被纳入后续的药物发现筛选中。
