Allary M, Saint-Blancard J, Boschetti E, Girot P
CTSA, Clamart, France.
Bioseparation. 1991;2(3):167-75.
Immobilized Cibacron Blue is a well-known tool for the separation of a number of proteins and enzymes. Among them, human plasma albumin was easily purified from crude plasma and consequently, this approach represents an interesting way for a production scale. Our data describes about three years of experience in the production of human albumin using large columns of immobilized Cibacron Blue. The amount of albumin produced per cycle was about 250 g on a column of about 50 l. Over 500 cycles the final purity of albumin was very high and constant (98-100%). The albumin yield of the chromatographic fractionation was approximately 82%. The column performance remained acceptable when regeneration operations were applied to the sorbent. The long life of the sorbent renders this approach very attractive and economically acceptable for large scale applications.
固定化汽巴蓝是分离多种蛋白质和酶的一种知名工具。其中，人血浆白蛋白很容易从粗血浆中纯化出来，因此，这种方法对于大规模生产来说是一种有趣的方式。我们的数据描述了使用大型固定化汽巴蓝柱生产人白蛋白约三年的经验。在一个约50升的柱上，每个循环产生的白蛋白量约为250克。在超过500个循环中，白蛋白的最终纯度非常高且恒定（98 - 100%）。色谱分离的白蛋白产率约为82%。当对吸附剂进行再生操作时，柱性能仍然可以接受。吸附剂的长寿命使得这种方法对于大规模应用非常有吸引力且在经济上可行。
