基质对白蛋白与固定化汽巴蓝结合的影响。
The effect of matrix on the binding of albumin to immobilized Cibacron Blue.
作者信息
Angal S, Dean P D
出版信息
Biochem J. 1977 Oct 1;167(1):301-3. doi: 10.1042/bj1670301.
Abstract
With Cibacron Blue as a ligand, the suitability of Sepharose, Sephacryl, cellulose and Ultrogel as column supports for the affinity chromatography of human serum albumin was examined. Sepharose and Sephacryl were the most effective matrices. Albumin was readily removed from such columns by using an electrophoretic desorption technique.
摘要
以汽巴蓝作为配体，研究了琼脂糖、葡聚糖凝胶、纤维素和优他胶作为柱支持物用于人血清白蛋白亲和层析的适用性。琼脂糖和葡聚糖凝胶是最有效的基质。通过电泳解吸技术可轻松从此类柱上除去白蛋白。
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