Soltés L, Mendichi R
Institute of Experimental Pharmacology, Slovak Academy of Sciences, SK-84104 Bratislava, Slovak Republic.
Biomed Chromatogr. 2003 Sep;17(6):376-84. doi: 10.1002/bmc.252.
Molecular characteristics were determined of two high-molecular-weight water-soluble hyaluronan derivatives, namely beta-cyclodextrin (HA-beta-CD) and N-acylurea (EDC-HA). The weight-average molecular weight (M(w)) of HA-beta-CD and of EDC-HA, determined with a multi-angle light scattering detector connected on-line to a size exclusion chromatographic system, was respectively 185.3 and 86.8 kDa. However the M(w) value determined for the equimolar mixture of the two HA derivatives equaled 556.0 kDa. Similarly, the gyration radius of the above equimolar mixture, Rg = 80.6 nm, was significantly greater than the values found for the single HA derivative, i.e. 40.2 nm for HA-beta-CD and 23.8 nm for EDC-HA. These data indicate that the two kinds of substituents, bound to the polymeric chains, form host-guest/inclusion complexes resulting in polymacromolecular associates/aggregates.
测定了两种高分子量水溶性透明质酸衍生物的分子特性，即β-环糊精（HA-β-CD）和N-酰脲（EDC-HA）。通过在线连接多角度光散射检测器的尺寸排阻色谱系统测定，HA-β-CD和EDC-HA的重均分子量（M(w)）分别为185.3 kDa和86.8 kDa。然而，两种HA衍生物等摩尔混合物的M(w)值为556.0 kDa。同样，上述等摩尔混合物的回转半径Rg = 80.6 nm，明显大于单一HA衍生物的回转半径值，即HA-β-CD为40.2 nm，EDC-HA为23.8 nm。这些数据表明，与聚合物链结合的两种取代基形成了主客体/包合物，导致多聚大分子缔合体/聚集体的形成。
