Soltés Ladislav, Valachová Katarína, Mendichi Raniero, Kogan Grigorij, Arnhold Juergen, Gemeiner Peter
Institute of Experimental Pharmacology, Slovak Academy of Sciences, SK-84104 Bratislava, Slovakia.
Carbohydr Res. 2007 Jun 11;342(8):1071-7. doi: 10.1016/j.carres.2007.02.018. Epub 2007 Feb 23.
An accurate molecular characterization, molar mass and size distributions, of 10 hyaluronan (HA) samples was performed by using a multi-angle light scattering detector connected on-line to a size exclusion chromatographic system. The dynamic viscosity eta of the HA solutions was investigated using a rotational viscometer. On monitoring the sample dynamic viscosity for up to 5h, a small however constant increase of the eta value was observed, indicating rheopectic behavior of all 10 HA solutions. Addition of ascorbic acid to the HA solutions caused significant changes in the rheological properties of the samples investigated. The change of eta values in the course of time was explained by the redox reactions (caused by the added ascorbate) that occur during the dynamic viscosity monitoring.
通过将多角度光散射检测器与尺寸排阻色谱系统在线连接，对10个透明质酸（HA）样品进行了准确的分子表征、摩尔质量和尺寸分布分析。使用旋转粘度计研究了HA溶液的动态粘度η。在监测样品动态粘度长达5小时的过程中，观察到η值有微小但持续的增加，表明所有10种HA溶液均表现出触变性。向HA溶液中添加抗坏血酸会导致所研究样品的流变性质发生显著变化。动态粘度监测过程中η值随时间的变化是由添加抗坏血酸盐引起的氧化还原反应所解释的。
