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通过火焰分光光度法测定生物体液和组织中的镁。

The determination of magnesium in biological fluids and tissues by flame spectrophotometry.

作者信息

ALCOCK N, MACINTYRE I, RADDE I

出版信息

J Clin Pathol. 1960 Nov;13(6):506-10. doi: 10.1136/jcp.13.6.506.

DOI:10.1136/jcp.13.6.506
PMID:13682196
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC480131/
Abstract

A flame spectrophotometric method for estimating magnesium in biological fluids and tissues is described. It may be applied to the Zeiss spectrophotometer PMQ II with flame attachment. Interferences are small, and recoveries from plasma and urine and from ashed faeces, soft tissue, and bone are satisfactory. Calcium can be measured on the same dilutions of plasma and urine and faecal ash. Sodium, potassium, calcium, and magnesium can be estimated on the same dilutions of soft tissue and bone ash. The plasma magnesium concentration of normal subjects falls within a narrow range. Differences between mean normal values previously reported may be partly attributed to the magnesium salt used in preparation of standard solutions.

摘要

本文描述了一种用于估算生物体液和组织中镁含量的火焰分光光度法。该方法可应用于配备火焰附件的蔡司PMQ II型分光光度计。干扰较小,血浆、尿液以及灰化粪便、软组织和骨骼中的回收率令人满意。血浆和尿液以及粪便灰分的相同稀释液可用于测量钙含量。软组织和骨灰分的相同稀释液可用于估算钠、钾、钙和镁的含量。正常受试者的血浆镁浓度处于较窄范围内。先前报道的平均正常值之间的差异可能部分归因于制备标准溶液时所用的镁盐。

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1
The determination of magnesium in biological fluids and tissues by flame spectrophotometry.通过火焰分光光度法测定生物体液和组织中的镁。
J Clin Pathol. 1960 Nov;13(6):506-10. doi: 10.1136/jcp.13.6.506.
2
The determination of magnesium in biological materials by flame photometry.通过火焰光度法测定生物材料中的镁。
J Clin Pathol. 1961 Jul;14(4):403-9. doi: 10.1136/jcp.14.4.403.
3
[Flame spectrophotometry and dosage of sodium, potassium, calcium and magnesium ions in plasma and in tissues].[火焰分光光度法与血浆及组织中钠、钾、钙和镁离子的测定剂量]
Bull Soc Chim Biol (Paris). 1956 Oct 8;38(7-8):1071-81.
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The estimation of magnesium in small biological samples by flame spectrophotometry.通过火焰分光光度法对小生物样本中的镁进行测定。
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A new principle applied to the determination of calcium in biological materials by flame photometry.一种应用于通过火焰光度法测定生物材料中钙的新原理。
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Mitt Geb Lebensmittelunters Hyg. 1955;46(6):540-68.
7
Validation of candidate reference methods based on ion chromatography for determination of total sodium, potassium, calcium and magnesium in serum through comparison with flame atomic emission and absorption spectrometry.通过与火焰原子发射和吸收光谱法比较,验证基于离子色谱法测定血清中总钠、钾、钙和镁的候选参考方法。
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Correction of sources of error in the estimation of sodium, potassium and calcium in biological fluids and tissues by flame spectrophotometry.火焰分光光度法校正生物体液和组织中钠、钾及钙含量测定的误差来源
Biochim Biophys Acta. 1952 Nov;9(5):576-7. doi: 10.1016/0006-3002(52)90211-4.
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[Determination of electrolytes in serum and plasma].[血清和血浆中电解质的测定]
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A method for determining the total magnesium, calcium, sodium and potassium contents of human platelets.一种测定人体血小板中总镁、钙、钠和钾含量的方法。
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1
Neonatal Fits Associated with Hypomagnesaemia.新生儿低镁血症相关惊厥
Arch Dis Child. 1965 Jun;40(211):286-90. doi: 10.1136/adc.40.211.286.
2
AN AUTOMATED METHOD FOR SERUM MAGNESIUM ESTIMATION.一种血清镁含量测定的自动化方法。
J Clin Pathol. 1964 Nov;17(6):644-8. doi: 10.1136/jcp.17.6.644.
3
Incidence of hypomagnesaemia in intestinal malabsorption.肠道吸收不良中低镁血症的发生率。
Br Med J. 1963 Jul 20;2(5350):141-4. doi: 10.1136/bmj.2.5350.141.
4
Sensitive methods for the titrimetric microdetermination of biological calcium and magnesium.用于生物钙和镁滴定微量测定的灵敏方法。
J Clin Pathol. 1963 May;16(3):252-5. doi: 10.1136/jcp.16.3.252.
5
A routine method for the determination of phosphoglucose isomerase activity in body fluid.一种测定体液中磷酸葡萄糖异构酶活性的常规方法。
J Clin Pathol. 1963 May;16(3):248-51. doi: 10.1136/jcp.16.3.248.
6
The metabolic effects of intestinal resection in man.人体肠道切除术后的代谢影响。
Postgrad Med J. 1961 Dec;37(434):725-39. doi: 10.1136/pgmj.37.434.725.
7
Plasma magnesium in health and disease.健康与疾病中的血浆镁。
J Clin Pathol. 1961 Jul;14(4):410-4. doi: 10.1136/jcp.14.4.410.
8
The determination of magnesium in biological materials by flame photometry.通过火焰光度法测定生物材料中的镁。
J Clin Pathol. 1961 Jul;14(4):403-9. doi: 10.1136/jcp.14.4.403.
9
Comparison of four methods for determining serum magnesium.四种测定血清镁方法的比较
J Clin Pathol. 1968 Mar;21(2):220. doi: 10.1136/jcp.21.2.220.
10
Biochemical and genetic studies in cystinuria: observations on double heterozygotes of genotype I-II.胱氨酸尿症的生化与遗传学研究:关于I-II型双杂合子的观察
J Clin Invest. 1971 Sep;50(9):1961-76. doi: 10.1172/JCI106688.

本文引用的文献

1
Quantitative determination of calcium and magnesium in human serum by flame spectrophotometry.火焰分光光度法对人血清中钙和镁的定量测定。
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The fluorometric estimation of magnesium in serum and in urine.血清和尿液中镁的荧光测定法。
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The production of secondary potassium depletion, sodium retention, nephrocalcinosis and hypercalcaemia by magnesium deficiency.镁缺乏导致继发性钾缺乏、钠潴留、肾钙质沉着症和高钙血症。
Biochem J. 1958 Nov;70(3):456-62. doi: 10.1042/bj0700456.
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Calcium and magnesium content of normal human blood serum.
Nature. 1958 Jul 26;182(4630):263-4. doi: 10.1038/182263a0.
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Estimation of magnesium in serum by means of flame spectrophotometry.用火焰分光光度法测定血清中的镁。
Am J Clin Pathol. 1958 Aug;30(2):129-32. doi: 10.1093/ajcp/30.2.129.
6
A study of magnesium metabolism in acute renal failure employing a multichannel flame spectrometer.
N Engl J Med. 1957 Dec 26;257(26):1254-62. doi: 10.1056/NEJM195712262572602.
7
The flame-spectrophotometric determination of calcium in biological fluids and an isotopic analysis of the errors in the Kramer-Tisdall procedure.生物体液中钙的火焰分光光度法测定及 Kramer-Tisdall 法误差的同位素分析。
Biochem J. 1957 Sep;67(1):164-72. doi: 10.1042/bj0670164.
8
Improved method for determination of calcium and magnesium in biologic fluids by EDTA titration.通过乙二胺四乙酸(EDTA)滴定法测定生物体液中钙和镁的改进方法。
Am J Clin Pathol. 1956 Oct;26(10):1157-68. doi: 10.1093/ajcp/26.10.1157.
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A flame photometric method for the determination of plasma magnesium after hydroxyquinoline precipitation.
J Biol Chem. 1955 Oct;216(2):643-51.