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通过火焰分光光度法测定生物体液和组织中的镁。

The determination of magnesium in biological fluids and tissues by flame spectrophotometry.

作者信息

ALCOCK N, MACINTYRE I, RADDE I

出版信息

J Clin Pathol. 1960 Nov;13(6):506-10. doi: 10.1136/jcp.13.6.506.

Abstract

A flame spectrophotometric method for estimating magnesium in biological fluids and tissues is described. It may be applied to the Zeiss spectrophotometer PMQ II with flame attachment. Interferences are small, and recoveries from plasma and urine and from ashed faeces, soft tissue, and bone are satisfactory. Calcium can be measured on the same dilutions of plasma and urine and faecal ash. Sodium, potassium, calcium, and magnesium can be estimated on the same dilutions of soft tissue and bone ash. The plasma magnesium concentration of normal subjects falls within a narrow range. Differences between mean normal values previously reported may be partly attributed to the magnesium salt used in preparation of standard solutions.

摘要

本文描述了一种用于估算生物体液和组织中镁含量的火焰分光光度法。该方法可应用于配备火焰附件的蔡司PMQ II型分光光度计。干扰较小,血浆、尿液以及灰化粪便、软组织和骨骼中的回收率令人满意。血浆和尿液以及粪便灰分的相同稀释液可用于测量钙含量。软组织和骨灰分的相同稀释液可用于估算钠、钾、钙和镁的含量。正常受试者的血浆镁浓度处于较窄范围内。先前报道的平均正常值之间的差异可能部分归因于制备标准溶液时所用的镁盐。

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本文引用的文献

4
Calcium and magnesium content of normal human blood serum.
Nature. 1958 Jul 26;182(4630):263-4. doi: 10.1038/182263a0.
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A study of magnesium metabolism in acute renal failure employing a multichannel flame spectrometer.
N Engl J Med. 1957 Dec 26;257(26):1254-62. doi: 10.1056/NEJM195712262572602.

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