Kawasaki Y, Isoda H, Tanimoto M, Dosako S, Idota T, Ahiko K
Technical Research Institute, Snow Brand Milk Products Co., Ltd., Kawagoe, Japan.
Biosci Biotechnol Biochem. 1992 Feb;56(2):195-8. doi: 10.1271/bbb.56.195.
Inhibition from binding of Cholera toxin (CT) to Chinese hamster ovary (CHO)-K1 cells and ganglioside GM1 by lactoferrin (Lf) and kappa-casein glycomacropeptide (GMP) from cow's milk was examined. Both Lf and GMP effectively reduced the CT-derived morphological changes in CHO-K1 cells. The competitive binding assay demonstrated that both Lf and GMP inhibited the binding of CT to GM1, although their affinity for CT was lower than that of GM1. The inhibitory effect of Lf and GMP seemed to be attributed to their terminal sialic acid, although the sugar chain sequence only partially fitted to the CT-receptor.
研究了乳铁蛋白（Lf）和牛乳中的κ-酪蛋白糖巨肽（GMP）对霍乱毒素（CT）与中国仓鼠卵巢（CHO）-K1细胞及神经节苷脂GM1结合的抑制作用。Lf和GMP均有效减轻了CT诱导的CHO-K1细胞形态变化。竞争性结合试验表明，Lf和GMP均抑制CT与GM1的结合，尽管它们对CT的亲和力低于GM1。Lf和GMP的抑制作用似乎归因于其末端唾液酸，尽管糖链序列仅部分符合CT受体。
