Idota T, Kawakami H, Murakami Y, Sugawara M
Technical Research Institute, Snow Brand Milk Products Co., Ltd., Saitama, Japan.
Biosci Biotechnol Biochem. 1995 Mar;59(3):417-9. doi: 10.1271/bbb.59.417.
The effects of human milk fractions on clolera toxin B subunit binding to monosialoganglioside 1 (GM1) were investigated. Human milk, human defatted milk, whey, and a low-molecular-weight fraction of human milk inhibited the binding, but casein did not inhibit it. The inhibitory activity of whey from bovine-milk-based infant formula was less than that of whey from human milk. Differences in composition between human and bovine whey seemed to influence the extent of the inhibitory activity. Sialylated oligosaccharides were considered to be the possible components that inhibited cholera toxin. The effects of sialyllactose, a predominant sialylated component of human milk, on cholera toxin-induced diarrhea were investigated by the rabbit intestinal loop method. Sialyllactose inhibited the cholera toxin inducing fluid accumulation, although neither sialic acid nor lactose had an effect on it. The results suggest that sialyllactose is responsible for the inhibitory activity of milk on cholera toxin.
研究了人乳各组分对霍乱毒素B亚基与单唾液酸神经节苷脂1（GM1）结合的影响。人乳、人脱脂乳、乳清和人乳的低分子量组分可抑制这种结合，但酪蛋白无此抑制作用。以牛乳为基础的婴儿配方奶粉中的乳清抑制活性低于人乳中的乳清。人乳清和牛乳清在成分上的差异似乎影响了抑制活性的程度。唾液酸化低聚糖被认为是抑制霍乱毒素的可能成分。采用兔肠袢法研究了人乳中主要的唾液酸化成分唾液乳糖对霍乱毒素诱导腹泻的影响。唾液乳糖可抑制霍乱毒素诱导的液体蓄积，而唾液酸和乳糖对此均无作用。结果表明，唾液乳糖是乳汁对霍乱毒素抑制活性的原因所在。
