Kusakabe I, Watanabe S, Morita R, Terahara M, Murakami K
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Biosci Biotechnol Biochem. 1992 Feb;56(2):233-7. doi: 10.1271/bbb.56.233.
An Actinomycete, strain K-128, which was isolated from soil, formed a transfer product when the strain was cultured in a medium containing both stevioside and curdlan. The transfer product in culture broth was separated by DIAION HP-20 column and TOYOPEARL HW-40F column chromatographies. From structural studies, the transfer product was identified as C13-O-beta-6(2)-beta-glucosylsophorosyl-C19-O-beta-glucopyranosyl steviol. This product was the first to be obtained by a culture of an Actinomycete.
从土壤中分离出的一株放线菌K-128，当在含有甜菊糖苷和凝胶多糖的培养基中培养时，会形成一种转移产物。培养肉汤中的转移产物通过DIAION HP-20柱和TOYOPEARL HW-40F柱色谱进行分离。通过结构研究，该转移产物被鉴定为C13-O-β-6(2)-β-葡萄糖基槐糖基-C19-O-β-吡喃葡萄糖基甜菊醇。该产物是通过放线菌培养首次获得的。
