Landt O, Zirpel-Giesebrecht M, Milde A, Hahn U
Abteilung Saenger, Institut für Kristallographie, Freie Universität Berlin, Germany.
J Biotechnol. 1992 Jun;24(2):189-94. doi: 10.1016/0168-1656(92)90122-p.
Purification of recombinant RNase T1 and its mutants has been improved by optimizing bacterial growth conditions, periplasmic fraction preparation and the use of a precolumn. The main part of the chromatographic separation could be automated due to the reproducibility of the procedure.
