ARMSTRONG J A, BRANSBY-WILLIAMS W R
Bull World Health Organ. 1961;24(4-5):427-35.
The authors describe the establishment and maintenance of a laboratory colony of Anopheles gambiae in Tanganyika, giving an account of the techniques adopted in order to overcome the problems encountered, as a guide to those who may be faced with similar difficulties. They also report on a series of observations on larvae, adults and the effect of temperature on mortality, fertilization, egg production and the gonotrophic cycle.The optimum constant temperature for adults and larvae was found to be 26.5 degrees C, at which the cycle of development from egg to pupa took about 9 days, with a total larval mortality of 5%. A complete life-cycle from egg to oviposition took 15 days. The pupal mortality was 1.5% and the average daily adult mortality was 1.5% for females and 1.8% for males. Peak fertilization was reached in 7 days. The average daily number of eggs produced per cage of 400 males and 400 females was 4000, with a total daily colony production of 130 000 eggs.
作者描述了在坦噶尼喀建立和维持冈比亚按蚊实验室种群的情况,介绍了为克服所遇到的问题而采用的技术,为可能面临类似困难的人提供指导。他们还报告了一系列关于幼虫、成虫以及温度对死亡率、受精、产卵和生殖营养周期影响的观察结果。发现成虫和幼虫的最佳恒定温度为26.5摄氏度,在此温度下,从卵到蛹的发育周期约为9天,幼虫总死亡率为5%。从卵到产卵的完整生命周期为15天。蛹的死亡率为1.5%,雌性成虫平均每日死亡率为1.5%,雄性为1.8%。7天达到受精高峰。每笼400只雄性和400只雌性每天平均产卵数为4000枚,种群每日总产卵量为130000枚。