Locher G, Sonnleitner B, Fiechter A
Institute of Biotechnology, ETH Zürich Hönggerberg, Switzerland.
J Biotechnol. 1992 Aug;25(1-2):23-53. doi: 10.1016/0168-1656(92)90108-l.
Bioprocesses are generally ill controlled. This is due to the fact that the measurement of relevant variables is difficult. Therefore, fundamental knowledge of metabolic interrelations is, at least in vivo, limited. In this article, some of the most important measurement techniques are reviewed in order to provide an evaluation of their current state. Emphasis is given to the underlying principles and on-line capability which allow to judge their importance and potential for exploitation resulting in well (maybe entirely) controlled bioprocesses in the future.
生物过程通常难以得到良好控制。这是因为相关变量的测量很困难。因此，至少在体内，代谢相互关系的基础知识是有限的。在本文中，对一些最重要的测量技术进行了综述，以便对其当前状态进行评估。重点关注其基本原理和在线能力，从而能够判断它们在未来实现良好（甚至可能完全）控制的生物过程中的重要性和开发潜力。
