用脉冲场凝胶电泳分离的DNA对酿酒酵母进行染色体转化。
Chromosomal transformation in Saccharomyces cerevisiae with DNA isolated by pulse field gel electrophoresis.
作者信息
Goto K, Motoyoshi T, Tamura G, Obata T, Hara S
机构信息
National Research Institute of Brewing, Tokyo, Japan.
出版信息
Agric Biol Chem. 1990 Jun;54(6):1499-504.
We isolated and purified yeast chromosome DNA molecules using pulse field gel electrophoresis (PFG). The isolated DNA had nearly the same size as the native chromosomal DNA on PFG. We could directly transform Saccharomyces cerevisiae yeasts with it, and obtain transformants that were selected by complementation of several markers. They had new chromosome DNA bands observed on PFG. The new chromosome was very stable during mitosis and mating processes, and each of the three homologous chromosomes in the derivative zygotes of transformants was separated equally in daughter cells.
我们使用脉冲场凝胶电泳（PFG）分离并纯化了酵母染色体DNA分子。在PFG上，分离得到的DNA与天然染色体DNA大小几乎相同。我们能够直接用它转化酿酒酵母，并获得通过几种标记互补筛选出的转化体。在PFG上观察到它们有新的染色体DNA条带。新染色体在有丝分裂和交配过程中非常稳定，并且转化体衍生合子中的三条同源染色体在子细胞中均能平等分离。
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