细胞固定化和有机溶剂对深黄被孢霉硫氧化和类固醇羟基化的影响。

Effect of cell immobilization and organic solvents on sulfoxidation and steroid hydroxylation by Mortierella isabellina.

作者信息

Holland H L, Poddar S, Tripet B

机构信息

Department of Chemistry, Brock University, St. Catharines, Ontario, Canada.

出版信息

J Ind Microbiol. 1992 Sep;10(3-4):195-7. doi: 10.1007/BF01569766.

DOI:10.1007/BF01569766

PMID:1368869

Abstract

The effects of calcium alginate bead immobilization and the presence of organic solvents on two bioconversion reactions carried out by Mortierella isabellina ATCC 42613 have been investigated. These reactions, the 14 alpha-hydroxylation of progesterone and the sulfoxidation of thioanisole, both proceed in high yield using resting-cell bioconversions, but are not carried out by alginate bead preparations in the absence of an organic co-solvent, the best results being obtained with 5 or 10% aqueous methanol. The stereoselectivity of sulfoxidation of thioanisole was found to be dependent upon the nature and concentration of organic co-solvent.

摘要

研究了海藻酸钙珠固定化以及有机溶剂的存在对深黄被孢霉ATCC 42613所进行的两个生物转化反应的影响。这两个反应，即孕酮的14α-羟基化反应和苯甲硫醚的亚砜化反应，使用静息细胞生物转化时均能高产率进行，但在没有有机共溶剂的情况下，海藻酸钙珠制剂无法进行这两个反应，在5%或10%的甲醇水溶液中可获得最佳结果。发现苯甲硫醚亚砜化反应的立体选择性取决于有机共溶剂的性质和浓度。