Department of Biological Engineering, Yosu National University, San 96-1, Doonduk-Dong, Yosu, Chunnam, 550-250, Korea.
Cytotechnology. 1999 Nov;31(3):265-70. doi: 10.1023/A:1008060502532.
The effect of oxygen partial pressure on viral replication was investigated with Vero/VSV system. At 10% oxygen partial pressure in spinner culture, VSV titer was significantly increased 130 fold compared to that obtained at 21%. A similar result was obtained for viral production in 1liter bioreactor. This implies that oxygen partial pressure during viral production has to be low. In low oxygen partial pressure, malondialdehyde concentration was decreased about 5 fold. Thus, low oxygen partial pressure allowed the reduced reactive oxygen species to be evolved, possibly by decreasing the random oxidation of the produced viral protein and membrane from the host cell.
研究了氧分压对病毒复制的影响，使用 Vero/VSV 系统。在旋转培养物中氧分压为 10%时，与 21%时相比，VSV 效价显著增加了 130 倍。在 1 升生物反应器中进行病毒生产时也得到了类似的结果。这意味着病毒生产过程中的氧分压必须低。在低氧分压下，丙二醛浓度降低了约 5 倍。因此，低氧分压允许减少的活性氧物质产生，可能是通过减少宿主细胞产生的病毒蛋白和膜的随机氧化。
