Dhainaut F, Bihoreau N, Meterreau J L, Lirochon J, Vincentelli R, Mignot G
Innovation Therapeutique, CNTS 3, Les Ulis, France.
Cytotechnology. 1992;10(1):33-41. doi: 10.1007/BF00376098.
The performance of a protein-free medium was compared in culture flasks with a serum-supplemented medium and with a serum free medium in terms of cell growth and monoclonal antibody production by a murine hybridoma. We present results of continuous production in hollow fiber culture systems using serum-free medium and protein-free medium. In protein-free medium, it has been possible to produce large quantities of monoclonal antibody with a productivity similar to that obtained in serum-free medium. After a two steps purification process, monoclonal antibodies were characterized by SDS-PAGE, High Performance Size Exclusion Chromatography and Free Solution Capillary Electrophoresis. SDS-PAGE and high performance chromatography analysis have showed that purified monoclonal antibodies produced in serum-free medium or protein-free medium were similar. Furthermore, Capillary Electrophoresis characterization revealed that both MAbs were constituted by three isoforms with equivalent electrophoretic mobilities.
就小鼠杂交瘤的细胞生长和单克隆抗体产生而言，在培养瓶中比较了无蛋白培养基与补充血清的培养基以及无血清培养基的性能。我们展示了使用无血清培养基和无蛋白培养基在中空纤维培养系统中连续生产的结果。在无蛋白培养基中，有可能产生大量单克隆抗体，其生产率与在无血清培养基中获得的生产率相似。经过两步纯化过程后，通过SDS-PAGE、高效尺寸排阻色谱法和自由溶液毛细管电泳对单克隆抗体进行了表征。SDS-PAGE和高效色谱分析表明，在无血清培养基或无蛋白培养基中产生的纯化单克隆抗体相似。此外，毛细管电泳表征显示，两种单克隆抗体均由三种具有等效电泳迁移率的同工型组成。
