薄层色谱法、高效液相色谱法和质谱法在抗生素尼博霉素发酵与分离中的应用

Applications of thin layer chromatography, high performance liquid chromatography and mass spectrometry in the fermentation and isolation of the antibiotic nybomycin.

作者信息

Pope J A, Nelson R A, Schaffner C P, Rosen R T, Pandey R C

机构信息

Waksman Institute of Microbiology, Rutgers-The State University of New Jersey, Piscataway.

出版信息

J Ind Microbiol. 1990 Sep;6(1):61-9. doi: 10.1007/BF01576178.

DOI:10.1007/BF01576178

PMID:1369285

Abstract

Thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and mass spectrometry (MS) methods have been developed for the analysis of the antibiotic nybomycin, its derivatives deoxynybomycin and nybomycin acetate, during the fermentation and isolation of nybomycin. Using a quantitative HPLC based assay, the time course of nybomycin production (nybomycin titers) in 1000 liter fermentations was determined. Desorption chemical ionization mass spectrometry (DCI/MS) of standard nybomycin samples, fermentation broth samples and purified fractions suggested the co-production of deoxynybomycin which was not reported previously from this organism. TLC and HPLC were used to confirm the presence of deoxynybomycin in the crude extracts of fermentation broths.

摘要

已开发出薄层色谱法（TLC）、高效液相色谱法（HPLC）和质谱法（MS），用于在尼博霉素发酵和分离过程中分析抗生素尼博霉素、其衍生物脱氧尼博霉素和乙酸尼博霉素。使用基于HPLC的定量测定法，测定了1000升发酵中尼博霉素的产生时间进程（尼博霉素效价）。标准尼博霉素样品、发酵液样品和纯化级分的解吸化学电离质谱（DCI/MS）表明同时产生了脱氧尼博霉素，此前该菌株尚未有此报道。TLC和HPLC用于确认发酵液粗提物中脱氧尼博霉素的存在。