Temeyer K B, Hopkins K M, Chapman L F
Agricultural Research Service, US Department of Agriculture, Kerrville, TX 78028.
SAAS Bull Biochem Biotechnol. 1991 Jan;4:52-5.
The recombinant DNA junctions at which pUB110 and Bacillus subtilis chromosomal DNA were joined to form the plasmid pKBT1 were cloned and sequenced. From the sequencing data we conclude that the pUB110 sequence is intact in the pair of cloned pKBT1 fragments and pTL12 sequences are not present. A molecular model for the formation of pKBT1 based on structural motifs characteristic of the joint sites is presented.
克隆并测序了pUB110与枯草芽孢杆菌染色体DNA连接形成质粒pKBT1的重组DNA连接位点。根据测序数据，我们得出结论：在一对克隆的pKBT1片段中，pUB110序列完整，且不存在pTL12序列。基于连接位点特征性结构基序，提出了一个pKBT1形成的分子模型。
