Karpova I S, Pidpala O V, Shul'zhenko V N, Kostetskiĭ I E, Koretskaia N V, Lukash L L
Tsitol Genet. 1994 Jan-Feb;28(1):66-73.
The method for testing foreign plasmid DNA mutagenicity on the competent culture of B. subtilis has been developed. High mutagenic effect of DNA of recombinant plasmids carrying a single human Alu-repeat or the same repeat in combination with human apoAi gene or human insulin gene was demonstrated. The vector plasmid pUC18 had no mutagenic activity. According to the data of dot-blotting some fragments of recombinant plasmid DNA of human origin can integrate in B. subtilis chromosome by means of illegitimate recombination. It is concluded that B. subtilis test system is suitable for detection of potential mutagenic polynucleotide sequences in recombinant plasmid constructions produced for gene therapy purposes.
已开发出在枯草芽孢杆菌感受态培养物上测试外源质粒DNA诱变性的方法。携带单个人类Alu重复序列或与人类载脂蛋白A1基因或人类胰岛素基因组合的相同重复序列的重组质粒DNA具有高诱变作用。载体质粒pUC18没有诱变活性。根据斑点杂交数据，一些源自人类的重组质粒DNA片段可通过非法重组整合到枯草芽孢杆菌染色体中。得出结论，枯草芽孢杆菌测试系统适用于检测为基因治疗目的而构建的重组质粒中潜在的诱变多核苷酸序列。
