CHOPPIN P W, TAMM I
J Exp Med. 1960 Nov 1;112(5):921-44. doi: 10.1084/jem.112.5.921.
Inhibitors present in normal human and animal sera prevented hemagglutination by and neutralized infectivity of inhibitor-sensitive influenza A2 virus. Starch zone electrophoresis of sera indicated that the same serum components possess both hemagglutination-inhibiting and neutralizing activities. The greatest amount of inhibitory activity was found in normal horse serum, and the inhibitory activity increased with heating or treatment with concentrated solutions of urea. The inhibitory activities on human, ferret, and rabbit sera were markedly reduced but not completely eliminated by V. cholerae filtrate and purified neuraminidase. The inhibitory activity of horse serum was only moderately reduced by these agents. The nature of the horse serum inhibitor and the differences in the interactions of inhibitor-sensitive and insensitive influenza A2 virus particles and pre-1957 influenza viruses with receptors have been discussed.
正常人血清和动物血清中的抑制剂可抑制甲型流感病毒A2的血凝反应,并中和其感染性。血清的淀粉区带电泳表明,相同的血清成分同时具有血凝抑制和中和活性。正常马血清中的抑制活性最高,且加热或用浓缩尿素溶液处理后,抑制活性增强。霍乱弧菌滤液和纯化的神经氨酸酶可显著降低人、雪貂和兔血清的抑制活性,但并未将其完全消除。这些试剂仅适度降低马血清的抑制活性。文中讨论了马血清抑制剂的性质,以及甲型流感病毒A2敏感株和不敏感株与1957年前流感病毒与受体相互作用的差异。