Sadana A
Chemical Engineering Department, University of Mississippi, University 38677-9740.
Bioseparation. 1992;3(5):297-320.
Protein inactivations at liquid-liquid, gas-liquid, and liquid-solid interfaces are presented. Wherever possible the mechanisms of protein inactivation, the extent of inactivation, and means by which this inactivation may be minimized are presented. Emphasis is placed on the 'quality' or the heterogeneity of the protein absorbed at the different types of interfaces. The analysis of the adsorption of proteins at different types of interfaces presented together provides novel physical insights into protein interactions at interfaces. The influence of protein adsorption at interfaces on bioseparations is analyzed by discussing examples on two-phase separations, fermentation systems, membrane separation systems, and chromatographic separations. Valuable knowledge gained during protein adsorption for biomedical applications may be applied with caution to bioseparation systems wherever appropriate. Future theoretical and experimental analysis on protein adsorption in bioseparation systems should pay more attention to the 'quality' of the protein adsorbed at the interface.
本文介绍了蛋白质在液-液、气-液和液-固界面的失活情况。文中尽可能阐述了蛋白质失活的机制、失活程度以及使这种失活最小化的方法。重点在于不同类型界面上吸附蛋白质的“质量”或异质性。综合分析蛋白质在不同类型界面上的吸附情况，为界面处蛋白质相互作用提供了全新的物理见解。通过讨论两相分离、发酵系统、膜分离系统和色谱分离等实例，分析了界面处蛋白质吸附对生物分离的影响。蛋白质吸附过程中获得的有价值的生物医学应用知识，可在适当情况下谨慎应用于生物分离系统。未来生物分离系统中蛋白质吸附的理论和实验分析应更加关注界面处吸附蛋白质的“质量”。
