利用噬菌体P1系统克隆高分子量DNA片段。

Cloning high molecular weight DNA fragments by the bacteriophage P1 system.

作者信息

Sternberg N L

机构信息

Cancer Biology Program, Du Pont Merck Pharmaceutical Company, Wilmington, DE 19880-0328, USA.

出版信息

Trends Genet. 1992 Jan;8(1):11-6. doi: 10.1016/0168-9525(92)90018-y.

DOI:10.1016/0168-9525(92)90018-y

PMID:1369729

Abstract

The cloning of high molecular weight genomic DNA promises to provide the means of mapping chromosomes, isolating genes, and understanding long-range effects on gene expression. This review describes the background and some recent advances in cloning of high molecular weight DNA using the bacteriophage P1 system.

摘要

高分子量基因组DNA的克隆有望提供绘制染色体图谱、分离基因以及了解对基因表达的长期影响的方法。这篇综述描述了使用噬菌体P1系统克隆高分子量DNA的背景和一些最新进展。