用单克隆抗体对牛莫拉菌血凝粘附素进行定量分析。
Quantification of Moraxella bovis haemagglutinating adhesins with monoclonal antibodies.
作者信息
Gil-Turnes C, Aleixo J A
机构信息
Universidade Federal de Pelotas, Centro de Biotecnologia, RS, Brazil.
出版信息
Lett Appl Microbiol. 1991 Aug;13(2):55-7. doi: 10.1111/j.1472-765x.1991.tb00569.x.
Six monoclonal antibodies (MAbs) against Moraxella bovis GF 9 were used to quantify haemagglutinating adhesins of 16 strains of this organism. The amount of each MAb necessary to inhibit one haemagglutinating unit of each strain varied between 4 and 0.007 times that required by strain GF 9. Five strains reacted with six MAbs, one with five, two with four, one with three, two with two and three with none. The procedures used enabled to detect dominant strains candidates for vaccines.
使用六种抗牛莫拉菌GF 9的单克隆抗体（MAb）对该菌的16个菌株的血凝黏附素进行定量。抑制每个菌株一个血凝单位所需的每种单克隆抗体的量在菌株GF 9所需量的4倍至0.007倍之间变化。五个菌株与六种单克隆抗体反应，一个与五种反应，两个与四种反应，一个与三种反应，两个与两种反应，三个与任何单克隆抗体都不反应。所采用的程序能够检测出候选疫苗的优势菌株。
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