Tateyama H, Eimoto T, Tada T, Niwa T
Department of Pathology, Nagoya City University School of Medicine, Japan.
Cancer. 1992 Jan 1;69(1):127-32. doi: 10.1002/1097-0142(19920101)69:1<127::aid-cncr2820690123>3.0.co;2-s.
A case is reported of malignant pilomatricoma confirmed by immunohistochemistry using anti-human hair keratin (anti-HHK) antibody prepared by the authors. The tumor occurred in the soft tissue of the inguinal region of an 88-year-old woman, with later invasion of the epidermis. No other possible primary lesion was found at autopsy. Histologically, the tumor was squamous cell carcinoma with nests of tumor cells and shadow cell-like necrotic cells showing central keratinization and focal calcification. Immunohistochemically, the hair keratin was positive in this tumor and in benign pilomatricomas exclusively. All other skin lesions and various squamous cell carcinomas examined were negative for this antigen. The staining patterns of commercial antiepidermal keratin and antiinvolucrin antibodies were significantly different from that of anti-HHK in normal skin and in these lesions. To the authors' knowledge, this is the first case of malignant pilomatricoma tested with anti-HHK staining. Malignant pilomatricoma is generally a low-grade malignant tumor, but it can metastasize and be fatal as it was in this case.
本文报道了一例经作者制备的抗人毛发角蛋白（anti-HHK）抗体免疫组化确诊的恶性毛母质瘤病例。该肿瘤发生于一名88岁女性腹股沟区的软组织，随后侵犯表皮。尸检未发现其他可能的原发灶。组织学上，肿瘤为鳞状细胞癌，有肿瘤细胞巢及类似影子细胞的坏死细胞，可见中央角化和局灶性钙化。免疫组化显示，毛发角蛋白在该肿瘤及良性毛母质瘤中呈阳性，而所有其他检查的皮肤病变及各种鳞状细胞癌对该抗原均为阴性。在正常皮肤和这些病变中，市售的抗表皮角蛋白和抗兜甲蛋白抗体的染色模式与anti-HHK明显不同。据作者所知，这是首例用anti-HHK染色检测的恶性毛母质瘤病例。恶性毛母质瘤通常是一种低级别恶性肿瘤，但它可能会转移并致命，本病例即是如此。
