Yoshikawa K, Katagata Y, Kondo S
Department of Dermatology, Yamagata University School of Medicine, Japan.
J Invest Dermatol. 1995 Mar;104(3):396-400. doi: 10.1111/1523-1747.ep12665888.
Specimens of trichilemmal cyst, malignant trichilemmoma, keratoacanthoma, and epidermal cyst were examined to characterize keratin peptides in hair-follicle-derived tumors. Keratins were extracted from the specimens and analyzed by two-dimensional gel electrophoresis and densitometry; the results were then compared with those for normal epidermis, the outer root sheath of hair follicles, psoriatic epidermis, and various nonfollicular cutaneous epithelial tumors. The specific nonfollicular tumors examined were squamous cell carcinoma, Bowen disease, actinic keratosis, eccrine porocarcinoma, and sebaceous carcinoma. Immunohistochemistry also was performed with a few anti-keratin monoclonal antibodies. As a general rule, K6 and K16 were expressed in hyperproliferative conditions, such as epidermal tumors, and K17 was coexpressed in the same lesions. The ratio of K16 to K17 in many epithelial skin tumors has been unclear until now. K17 content exceeded K16 content in most follicular tumors, whereas in almost all the nonfollicular tumors and the psoriatic epidermis, K17 levels were less than or about equal to K16 levels. There was a significant difference in the ratio of K16 to K17 between follicular and nonfollicular skin tumors. These results indicate that alterations in the content of these keratins may be associated with follicular differentiation.
对毛鞘囊肿、恶性毛鞘瘤、角化棘皮瘤和表皮囊肿的标本进行检查，以鉴定毛囊源性肿瘤中的角蛋白肽。从标本中提取角蛋白，通过二维凝胶电泳和光密度测定法进行分析；然后将结果与正常表皮、毛囊外根鞘、银屑病表皮以及各种非毛囊性皮肤上皮肿瘤的结果进行比较。所检查的特定非毛囊性肿瘤包括鳞状细胞癌、鲍恩病、光化性角化病、小汗腺汗孔癌和皮脂腺癌。还用几种抗角蛋白单克隆抗体进行了免疫组织化学检测。一般来说，K6和K16在增殖过度的情况下表达，如表皮肿瘤，而K17在相同病变中共同表达。到目前为止，许多上皮性皮肤肿瘤中K16与K17的比例尚不清楚。在大多数毛囊性肿瘤中，K17含量超过K16含量，而在几乎所有非毛囊性肿瘤和银屑病表皮中，K17水平低于或约等于K16水平。毛囊性和非毛囊性皮肤肿瘤之间K16与K17的比例存在显著差异。这些结果表明，这些角蛋白含量的改变可能与毛囊分化有关。
