Marconi R T, Garon C F
Laboratory of Vectors and Pathogens, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840.
J Bacteriol. 1992 Jan;174(1):241-4. doi: 10.1128/jb.174.1.241-244.1992.
We have sequenced the 16S rRNA molecules from four species of Borrelia and from six isolates of Borrelia burgdorferi via the reverse transcriptase primer extension method. The sequences were aligned and evolutionary relationships were determined, including the calculation of evolutionary distances and the construction of a phylogenetic tree. These analyses demonstrate significant divergence among B. burgdorferi isolates, with the European isolates G1 and G2 residing most distant from the main cluster. Signature nucleotides which distinguish B. burgdorferi from all other members of this genus and which distinguish the European isolates G1 and G2 from the North American isolates B31, Sh-2-82, and 1352 were identified. Finally, Southern blot analyses were performed to compare the restriction patterns of the genes coding for rRNA and to relate our data to the grouping scheme of Postic et al. (D. Postic, C. Edlinger, C. Richaud, F. Grimont, J. Dufresne, P. Perolat, G. Baranton, and P. A. D. Grimont, Res. Microbiol. 141:465-475, 1990).
我们通过逆转录酶引物延伸法对四种疏螺旋体以及六株伯氏疏螺旋体的16S rRNA分子进行了测序。对这些序列进行比对并确定进化关系，包括计算进化距离和构建系统发育树。这些分析表明，伯氏疏螺旋体分离株之间存在显著差异，欧洲分离株G1和G2与主要聚类的距离最远。鉴定出了将伯氏疏螺旋体与该属所有其他成员区分开来以及将欧洲分离株G1和G2与北美分离株B31、Sh-2-82和1352区分开来的特征性核苷酸。最后，进行了Southern印迹分析，以比较编码rRNA的基因的限制性图谱，并将我们的数据与Postic等人（D. Postic、C. Edlinger、C. Richaud、F. Grimont、J. Dufresne、P. Perolat、G. Baranton和P. A. D. Grimont，《微生物学研究》141:465 - 475，1990年）的分组方案相关联。
