Lidin B, Lamon E W
Birmingham V.A. Medical Center, University of Alabama 35294.
Antiviral Res. 1992 Jan;17(1):79-89. doi: 10.1016/0166-3542(92)90092-j.
Two human Burkitt's lymphoma (BL) cell lines, Raji and Daudi, have been previously characterized as resistant and sensitive, respectively, to the anti-Epstein-Barr virus (EBV) effects of human leukocyte interferon. These cells are equally susceptible to P3HR-1 EBV superinfection as determined by EBV early antigen (EA) expression. The cell lines were pretreated with human recombinant interferons alpha 2, beta, or gamma and subsequently superinfected with P3HR-1 EBV. Their expression of two distinct EBV early gene products was evaluated by fluorescence microscopy. Monoclonal antibodies to the diffuse (EA-D) and restricted (EA-R) components of the EA complex were used to determine the number of cells expressing each of these antigens in the treated cell lines. As previously described with human leukocyte interferon, EA-D expression in Raji cells was relatively resistant to interferon-alpha 2 pretreatment. Also, EA-D expression in Daudi cells was relatively sensitive. However, interferon alpha 2 pretreatment produced an opposite pattern with respect to the expression of EA-R in these two cell lines; Raji cells were sensitive and Daudi cells relatively resistant. Interferon beta had the most uniformly effective anti-EBV activity on both cell lines; less than 15 U/ml produced 50% inhibition of both antigens in both cell lines. EA-D expression in both cell lines was sensitive to interferon-gamma pretreatment and EA-R was resistant. These data suggest that different gene products of EBV are independently regulated by interferons based on at least three factors: (1) the host cell, (2) the type of interferon and (3) the affected gene product.
两种人类伯基特淋巴瘤（BL）细胞系，拉吉（Raji）细胞系和道迪（Daudi）细胞系，先前已分别被鉴定为对人白细胞干扰素的抗爱泼斯坦 - 巴尔病毒（EBV）作用具有抗性和敏感性。通过EBV早期抗原（EA）表达确定，这些细胞对P3HR - 1 EBV超感染同样敏感。用重组人干扰素α2、β或γ对细胞系进行预处理，随后用P3HR - 1 EBV进行超感染。通过荧光显微镜评估它们两种不同EBV早期基因产物的表达。使用针对EA复合物的弥散性（EA - D）和局限性（EA - R）成分的单克隆抗体来确定在处理后的细胞系中表达每种这些抗原的细胞数量。如先前用人白细胞干扰素所描述的那样，拉吉细胞中EA - D的表达对干扰素α2预处理相对抗性。同样，道迪细胞中EA - D的表达相对敏感。然而，干扰素α2预处理在这两种细胞系中关于EA - R的表达产生了相反的模式；拉吉细胞敏感而道迪细胞相对抗性。干扰素β对两种细胞系都具有最一致有效的抗EBV活性；低于15 U/ml对两种细胞系中的两种抗原都产生50%的抑制。两种细胞系中EA - D的表达对干扰素γ预处理敏感而EA - R抗性。这些数据表明，EBV的不同基因产物基于至少三个因素受干扰素独立调节：（1）宿主细胞，（2）干扰素类型，以及（3）受影响的基因产物。
